Sequencing instruments by number
A quick one: I made this plot earlier today for a presentation. The data is from our Omicsmaps site which I curate with James Hadfield. Didn’t actually use it in the end but thought I’d post it in case it was useful for someone. Notable is the rise in HiSeq reciprocated by the decline in [...]
Adaptor trim or die: Experiences with Nextera libraries
One of the first posts I did on this blog, way back in September 2009 was about my experiences with filtering and trimming Illumina sequences, and it proved rather popular. To date, it has been viewed a whopping 8,560 times! But funnily enough, since that post was written my attitude towards filtering Illumina data slowly [...]
Balti and Bioinformatics: Midlands Sequencing and Bioinformatics Meeting: Monday, 20th May 2013
It’s that time again! Balti and bioinformatics will return .. on Monday, 20th May 2013. Not dissimilar to last time, we will feature complimentary samosas from Smethwick’s finest sweet shop, and hopefully without the bit where about 40 people had to crowd into my office. Spaces will be limited, please register to attend via the [...]
Applied Bioinformatics & Public Health Microbiology: 15 – 17 May 2013
The awesome ABPHM meeting is back in 2013! This is a really nice conference that I am very happy to help organise. It’s a bit different from other public health microbiology conferences in that it specifically aims to bring together public health microbiologists and epidemiologists with bioinformaticians. Once we have everyone in the same room, [...]
Sequencing data: I want the truth! (You can’t handle the truth!)
Two sequencing papers caught my eye this week. This letter from Piskol and Li is perhaps the final nail in the coffin for the heavily criticised and debunked (also see: GenomesUnzipped) RNA editing paper from Li and Cheung published in Science in early 2011 (as Thomas Keane said on Twitter: ‘I can’t believe people are still debating this!). The letter Piskol and Li examined [...]
Whole-genome sequencing for MRSA epidemiology: Transmission and “clouds of variation”
It’s an unusual sensation to wake up in the morning and hear Moira Stewart on the Radio 2 breakfast show talking about bacterial genomics and whole-genome sequencing. But it wasn’t a lucid dream, the publication of a new paper from Simon Harris and Sharon Peacock (of Cambridge University and the Sanger Centre, respectively) in Lancet [...]
Would you be interested in attending a regular, informal meeting on sequencing and bioinformatics in the Midlands?
Something I’ve wanted to do for ages in the Midlands since being inspired by Scotland’s excellent Nextgenbug series, please register below if you are interested:
Properly awesome: HiSeq 2500 2×151 rapid run streaming to BaseSpace
OK, I think this is awesome enough to share with you guys. These are some metagenomics samples being run on a HiSeq 2500 in 2x151bp rapid run mode, with the results being streamed to BaseSpace in real-time. What I love about this is that the various statistics and metrics update in real-time. I spent a [...]